|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat HSPD1 ORF mammalian expression plasmid, C-GFPSpark tag||RG81014-ACG|
|Rat HSPD1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81014-ACR|
|Rat HSPD1 ORF mammalian expression plasmid, N-GFPSpark tag||RG81014-ANG|
|Rat HSPD1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG81014-ANR|
|Rat HSPD1 ORF mammalian expression plasmid, C-Flag tag||RG81014-CF|
|Rat HSPD1 ORF mammalian expression plasmid, C-His tag||RG81014-CH|
|Rat HSPD1 ORF mammalian expression plasmid, C-Myc tag||RG81014-CM|
|Rat HSPD1 ORF mammalian expression plasmid, C-HA tag||RG81014-CY|
|Rat HSPD1 ORF mammalian expression plasmid, N-Flag tag||RG81014-NF|
|Rat HSPD1 ORF mammalian expression plasmid, N-His tag||RG81014-NH|
|Rat HSPD1 ORF mammalian expression plasmid, N-Myc tag||RG81014-NM|
|Rat HSPD1 ORF mammalian expression plasmid, N-HA tag||RG81014-NY|
|Rat HSPD1 Gene cDNA clone plasmid||RG81014-U|
|Rat HSPD1 natural ORF mammalian expression plasmid||RG81014-UT|
|Learn more about expression Vectors|
HSPD1, also known as HSP60, is a member of the chaperonin family. HSPD1 may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. It may also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. HSPD1 gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13). Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4); also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. HSPD1 is cinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.