|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse AK4 ORF mammalian expression plasmid, C-GFPSpark tag||MG52601-ACG|
|Mouse AK4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52601-ACR|
|Mouse AK4 ORF mammalian expression plasmid, N-GFPSpark tag||MG52601-ANG|
|Mouse AK4 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52601-ANR|
|Mouse AK4 ORF mammalian expression plasmid, C-Flag tag||MG52601-CF|
|Mouse AK4 ORF mammalian expression plasmid, C-His tag||MG52601-CH|
|Mouse AK4 ORF mammalian expression plasmid, C-Myc tag||MG52601-CM|
|Mouse AK4 ORF mammalian expression plasmid, C-HA tag||MG52601-CY|
|Mouse AK4 Gene cDNA clone plasmid||MG52601-G|
|Mouse AK4 ORF mammalian expression plasmid, N-Flag tag||MG52601-NF|
|Mouse AK4 ORF mammalian expression plasmid, N-His tag||MG52601-NH|
|Mouse AK4 ORF mammalian expression plasmid, N-Myc tag||MG52601-NM|
|Mouse AK4 ORF mammalian expression plasmid, N-HA tag||MG52601-NY|
|Mouse AK4 natural ORF mammalian expression plasmid||MG52601-UT|
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Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member the adenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.