|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat GPD1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80977-ACG|
|Rat GPD1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80977-ACR|
|Rat GPD1 ORF mammalian expression plasmid, N-GFPSpark tag||RG80977-ANG|
|Rat GPD1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80977-ANR|
|Rat GPD1 ORF mammalian expression plasmid, C-Flag tag||RG80977-CF|
|Rat GPD1 ORF mammalian expression plasmid, C-His tag||RG80977-CH|
|Rat GPD1 ORF mammalian expression plasmid, C-Myc tag||RG80977-CM|
|Rat GPD1 ORF mammalian expression plasmid, C-HA tag||RG80977-CY|
|Rat GPD1 ORF mammalian expression plasmid, N-Flag tag||RG80977-NF|
|Rat GPD1 ORF mammalian expression plasmid, N-His tag||RG80977-NH|
|Rat GPD1 ORF mammalian expression plasmid, N-Myc tag||RG80977-NM|
|Rat GPD1 ORF mammalian expression plasmid, N-HA tag||RG80977-NY|
|Rat GPD1 Gene cDNA clone plasmid||RG80977-U|
|Rat GPD1 natural ORF mammalian expression plasmid||RG80977-UT|
|Learn more about expression Vectors|
GPD1, also known as glycerolphosphate dehydrogenase 1, is a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. GPD1 catalyzes the reversible redox conversion of dihydroxyacetone phosphate (DHAP), thus plays a critical role in carbohydrate and lipid metabolism. It also reduces nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. Meanwhile, GPD1 and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in GPD1 gene are a cause of transient infantile hypertriglyceridemia.