|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat RAC2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80966-ACG|
|Rat RAC2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80966-ACR|
|Rat RAC2 ORF mammalian expression plasmid, N-GFPSpark tag||RG80966-ANG|
|Rat RAC2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80966-ANR|
|Rat RAC2 ORF mammalian expression plasmid, C-Flag tag||RG80966-CF|
|Rat RAC2 ORF mammalian expression plasmid, C-His tag||RG80966-CH|
|Rat RAC2 ORF mammalian expression plasmid, C-Myc tag||RG80966-CM|
|Rat RAC2 ORF mammalian expression plasmid, C-HA tag||RG80966-CY|
|Rat RAC2 ORF mammalian expression plasmid, N-Flag tag||RG80966-NF|
|Rat RAC2 ORF mammalian expression plasmid, N-His tag||RG80966-NH|
|Rat RAC2 ORF mammalian expression plasmid, N-Myc tag||RG80966-NM|
|Rat RAC2 ORF mammalian expression plasmid, N-HA tag||RG80966-NY|
|Rat RAC2 Gene cDNA clone plasmid||RG80966-U|
|Rat RAC2 natural ORF mammalian expression plasmid||RG80966-UT|
|Learn more about expression Vectors|
Ras-related C3 botulinum toxin substrate 2 (Rac2) is a small G-protein belonging to the Ras subfamily of the GTPase family. Rac2 acts as an "on / off" switch for signal transduction cascades and motilities. When GDP is attached to the small G-protein, the enzyme is inactivated. Release of the GDP and replace of the GTP cativate the GTPasee. Rac2 remains active until the GTP is hydrolyzed to GDP. Rac2 is a hematopoietic-specific Rho family GTPase implicated as an important constituent of the NADPH oxidase complex and shares 92% amino acid identity with the ubiquitously expressed Rac1. The small G-protein Rac2 regulates the rearrangements of actin and membrane necessary for Fcy receptor-mediated phagocytosis by macrophages. Activated Rac2 binds to the p21-binding domain of PAK1 and this binding provided a basis for microscopic methods to localize activation of these G proteins inside cells.