|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CPB2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80947-ACG|
|Rat CPB2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80947-ACR|
|Rat CPB2 ORF mammalian expression plasmid, C-Flag tag||RG80947-CF|
|Rat CPB2 ORF mammalian expression plasmid, C-His tag||RG80947-CH|
|Rat CPB2 ORF mammalian expression plasmid, C-Myc tag||RG80947-CM|
|Rat CPB2 ORF mammalian expression plasmid, C-HA tag||RG80947-CY|
|Rat CPB2 ORF mammalian expression plasmid, N-Flag tag||RG80947-NF|
|Rat CPB2 ORF mammalian expression plasmid, N-His tag||RG80947-NH|
|Rat CPB2 ORF mammalian expression plasmid, N-Myc tag||RG80947-NM|
|Rat CPB2 ORF mammalian expression plasmid, N-HA tag||RG80947-NY|
|Rat CPB2 Gene cDNA clone plasmid||RG80947-U|
|Rat CPB2 natural ORF mammalian expression plasmid||RG80947-UT|
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Carboxypeptidase B2, also known as Carboxypeptidase U, Thrombin-activable fibrinolysis inhibitor, Plasma carboxypeptidase B, CPB2, is a secreted protein which belongs to the peptidase M14 family. Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by thrombin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin / thrombomodulin complex. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. CPB2 exhibits carboxypeptidase activity and activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.