|Datasheet||Specific References||Reviews||Related Products||Protocols|
|A DNA sequence encoding the human NKG2D (Phe78-Val216) was fused with a polyhistide tag at the N-terminus.|
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|> 90 % as determined by SDS-PAGE|
|1. Immobilized human His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human ULBP1-Fch (Cat:10679-H03H), The EC50 of human ULBP1-Fch (Cat:10679-H03H) is 0.04-0.08 μg/ml.|
2. Immobilized human His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human MICB-Fch (Cat:10759-H03H), The EC50 of human MICB-Fch (Cat:10759-H03H) is 15.9-37.1 ng/ml.
|< 1.0 EU per μg of the protein as determined by the LAL method|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|The recombinant human NKG2D consists of 157 amino acids and has a calculated molecular mass of 18.4 kDa.|
|Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
NKG2D, also known as CD314, is an immune receptor which consists of two disulphide-linked type II transmembrane proteins with short intracellular proteins uncapable to transduce signals. In order to transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.