|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat FSTL1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80885-ACG|
|Rat FSTL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80885-ACR|
|Rat FSTL1 ORF mammalian expression plasmid, C-Flag tag||RG80885-CF|
|Rat FSTL1 ORF mammalian expression plasmid, C-His tag||RG80885-CH|
|Rat FSTL1 ORF mammalian expression plasmid, C-Myc tag||RG80885-CM|
|Rat FSTL1 ORF mammalian expression plasmid, C-HA tag||RG80885-CY|
|Rat FSTL1 ORF mammalian expression plasmid, N-Flag tag||RG80885-NF|
|Rat FSTL1 ORF mammalian expression plasmid, N-His tag||RG80885-NH|
|Rat FSTL1 ORF mammalian expression plasmid, N-Myc tag||RG80885-NM|
|Rat FSTL1 ORF mammalian expression plasmid, N-HA tag||RG80885-NY|
|Rat FSTL1 Gene cDNA clone plasmid||RG80885-U|
|Rat FSTL1 natural ORF mammalian expression plasmid||RG80885-UT|
|Learn more about expression Vectors|
Follistatin-related protein 1 (FSTL1) is an extracellular glycoprotein whose functional significance in physiological and pathological processes is incompletely understood. Recently, we have shown that FSTL1 acts as a muscle-derived secreted factor that is up-regulated by Akt activation and ischemic stress and that FSTL1 exerts favorable actions on the heart and vasculature. Here, we sought to identify the receptor that mediates the cellular actions of FSTL1. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. FSTL1 is thought to be an autoantigen associated with rheumatoid arthritis. DIP2A functions as a novel receptor that mediates the cardiovascular protective effects of FSTL1. Experiment results have provided in vivo and in vitro evidence to demonstrate that Fstl1 modulates lung development and alveolar maturation, in part, through BMP4 signaling.