|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse SOD1 ORF mammalian expression plasmid, C-GFPSpark tag||MG52544-ACG|
|Mouse SOD1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52544-ACR|
|Mouse SOD1 ORF mammalian expression plasmid, N-GFPSpark tag||MG52544-ANG|
|Mouse SOD1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52544-ANR|
|Mouse SOD1 ORF mammalian expression plasmid, C-Flag tag||MG52544-CF|
|Mouse SOD1 ORF mammalian expression plasmid, C-His tag||MG52544-CH|
|Mouse SOD1 ORF mammalian expression plasmid, C-Myc tag||MG52544-CM|
|Mouse SOD1 ORF mammalian expression plasmid, C-HA tag||MG52544-CY|
|Mouse SOD1 Gene cDNA clone plasmid||MG52544-G|
|Mouse SOD1 ORF mammalian expression plasmid, N-Flag tag||MG52544-NF|
|Mouse SOD1 ORF mammalian expression plasmid, N-His tag||MG52544-NH|
|Mouse SOD1 ORF mammalian expression plasmid, N-Myc tag||MG52544-NM|
|Mouse SOD1 ORF mammalian expression plasmid, N-HA tag||MG52544-NY|
|Mouse SOD1 natural ORF mammalian expression plasmid||MG52544-UT|
|Learn more about expression Vectors|
SOD1 belongs to the Cu-Zn superoxide dismutase family. It binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. SOD1 destroys radicals which are normally produced within the cells and which are toxic to biological systems. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1). ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.