|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CKM ORF mammalian expression plasmid, C-GFPSpark tag||RG80832-ACG|
|Rat CKM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80832-ACR|
|Rat CKM ORF mammalian expression plasmid, N-GFPSpark tag||RG80832-ANG|
|Rat CKM ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80832-ANR|
|Rat CKM ORF mammalian expression plasmid, C-Flag tag||RG80832-CF|
|Rat CKM ORF mammalian expression plasmid, C-His tag||RG80832-CH|
|Rat CKM ORF mammalian expression plasmid, C-Myc tag||RG80832-CM|
|Rat CKM ORF mammalian expression plasmid, C-HA tag||RG80832-CY|
|Rat CKM ORF mammalian expression plasmid, N-Flag tag||RG80832-NF|
|Rat CKM ORF mammalian expression plasmid, N-His tag||RG80832-NH|
|Rat CKM ORF mammalian expression plasmid, N-Myc tag||RG80832-NM|
|Rat CKM ORF mammalian expression plasmid, N-HA tag||RG80832-NY|
|Rat CKM Gene cDNA clone plasmid||RG80832-U|
|Rat CKM natural ORF mammalian expression plasmid||RG80832-UT|
|Learn more about expression Vectors|
CKM, also known as CK-MM, is mainly expressed in skeletal muscle. As the primary CK isoenzyme, it also can be detected in heart muscle. CKM is a subunit of creatine kinase (CK). CK is an enzyme expressed by various tissues and cell types. It catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In the cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type).