|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CD9 ORF mammalian expression plasmid, C-GFPSpark tag||RG80778-ACG|
|Rat CD9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80778-ACR|
|Rat CD9 ORF mammalian expression plasmid, C-Flag tag||RG80778-CF|
|Rat CD9 ORF mammalian expression plasmid, C-His tag||RG80778-CH|
|Rat CD9 ORF mammalian expression plasmid, C-Myc tag||RG80778-CM|
|Rat CD9 ORF mammalian expression plasmid, C-HA tag||RG80778-CY|
|Rat CD9 ORF mammalian expression plasmid, N-Flag tag||RG80778-NF|
|Rat CD9 ORF mammalian expression plasmid, N-His tag||RG80778-NH|
|Rat CD9 ORF mammalian expression plasmid, N-Myc tag||RG80778-NM|
|Rat CD9 ORF mammalian expression plasmid, N-HA tag||RG80778-NY|
|Rat CD9 Gene cDNA clone plasmid||RG80778-U|
|Rat CD9 natural ORF mammalian expression plasmid||RG80778-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD9 is a member of the transmembrane 4 superfamily, which is also known as the tetraspanin family. CD9 is a cell surface glycoprotein with 4 hydrophobic domains that is described to complex with integrins and other transmembrane 4 superfamily members. It is found expressed on the surface of the exosomes. The protein takes part in cellular signal transduction events and thus play a role in the regulation of cell development and activation, growth and motility. Besides, CD9 seems to be a key role in the egg-sperm fusion during the mammalian fertilization processes. CD9 is found on the membrane of the oocytes and also appears to intervene in maintaining the normal shape of oocyte microvilli.