|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat PSME1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80765-ACG|
|Rat PSME1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80765-ACR|
|Rat PSME1 ORF mammalian expression plasmid, N-GFPSpark tag||RG80765-ANG|
|Rat PSME1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80765-ANR|
|Rat PSME1 ORF mammalian expression plasmid, C-Flag tag||RG80765-CF|
|Rat PSME1 ORF mammalian expression plasmid, C-His tag||RG80765-CH|
|Rat PSME1 ORF mammalian expression plasmid, C-Myc tag||RG80765-CM|
|Rat PSME1 ORF mammalian expression plasmid, C-HA tag||RG80765-CY|
|Rat PSME1 ORF mammalian expression plasmid, N-Flag tag||RG80765-NF|
|Rat PSME1 ORF mammalian expression plasmid, N-His tag||RG80765-NH|
|Rat PSME1 ORF mammalian expression plasmid, N-Myc tag||RG80765-NM|
|Rat PSME1 ORF mammalian expression plasmid, N-HA tag||RG80765-NY|
|Rat PSME1 Gene cDNA clone plasmid||RG80765-U|
|Rat PSME1 natural ORF mammalian expression plasmid||RG80765-UT|
|Learn more about expression Vectors|
PA28A, also known as PSME1, is a subunit of proteasome. The 26S proteasome multicatalytic proteinase complex has a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME1 gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring.