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Human CA5A / CA-VA Protein (His Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Human CA5A Protein Product Information
Synonym:CA5, CAV, CAVA
Protein Construction:A DNA sequence encoding the mature form of human CA5A (NP_001730.1) (Ala 40-Ser 305) was fused with an Met at N-terminus and a polyhistide tag at the C-terminus.
Expressed Host:E. coli
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human CA5A Protein QC Testing
Purity:> 96 % as determined by SDS-PAGE
Bio-Activity:Measured by its esterase activity. The specific activity is >500 pmoles/min/μg.
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human CA5A consisting of 277 amino acids and has a calculated molecular mass of 31.6 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 50mM NaAc, 50mM NaCl, 0.05% Brij 35, pH 5.0
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human CA5A Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other CA5A Recombinant Protein Products
Carbonic Anhydrase VA Background

Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of the alpha-carbonic anhydrase family. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).

Human Carbonic Anhydrase VA References
  • Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 903.
  • Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262.
  • Temperini al., 2006, Chemistry 12: 7057-66.
  • Temperini al., 2006, J. Med. Chem. 49: 3019-27.
  • Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602.
  • Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.
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    Catalog: 10500-H08E-10
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