|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat F2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80722-ACG|
|Rat F2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80722-ACR|
|Rat F2 ORF mammalian expression plasmid, C-Flag tag||RG80722-CF|
|Rat F2 ORF mammalian expression plasmid, C-His tag||RG80722-CH|
|Rat F2 ORF mammalian expression plasmid, C-Myc tag||RG80722-CM|
|Rat F2 ORF mammalian expression plasmid, C-HA tag||RG80722-CY|
|Rat F2 ORF mammalian expression plasmid, N-Flag tag||RG80722-NF|
|Rat F2 ORF mammalian expression plasmid, N-His tag||RG80722-NH|
|Rat F2 ORF mammalian expression plasmid, N-Myc tag||RG80722-NM|
|Rat F2 ORF mammalian expression plasmid, N-HA tag||RG80722-NY|
|Rat F2 Gene cDNA clone plasmid||RG80722-U|
|Rat F2 natural ORF mammalian expression plasmid||RG80722-UT|
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Coagulation Factor II Protein (FII, F2 Protein or Prothrombin) is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. Coagulation Factor II Protein (FII, F2 Protein) also plays a role in maintaining vascular integrity during development and postnatal life. Prothrombin / Coagulation Factor II is activated on the surface of a phospholipid membrane that binds the amino end of prothrombin / Coagulation Factor II and factor Va and Xa in Ca-dependent interactions; factor Xa removes the activation peptide and cleaves the remaining part into light and heavy chains. The activation process starts slowly because factor V itself has to be activated by the initial, small amounts of thrombin. Prothrombin / Coagulation Factor II is expressed by the liver and secreted in plasma. Defects in prothrombin / Coagulation Factor II are the cause of factor II deficiency (FA2D). It is very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Defects in Coagulation Factor II are also a cause of susceptibility to thrombosis. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.