|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CCL6 ORF mammalian expression plasmid, C-GFPSpark tag||RG80721-ACG|
|Rat CCL6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80721-ACR|
|Rat CCL6 ORF mammalian expression plasmid, C-Flag tag||RG80721-CF|
|Rat CCL6 ORF mammalian expression plasmid, C-His tag||RG80721-CH|
|Rat CCL6 ORF mammalian expression plasmid, C-Myc tag||RG80721-CM|
|Rat CCL6 ORF mammalian expression plasmid, C-HA tag||RG80721-CY|
|Rat CCL6 ORF mammalian expression plasmid, N-Flag tag||RG80721-NF|
|Rat CCL6 ORF mammalian expression plasmid, N-His tag||RG80721-NH|
|Rat CCL6 ORF mammalian expression plasmid, N-Myc tag||RG80721-NM|
|Rat CCL6 ORF mammalian expression plasmid, N-HA tag||RG80721-NY|
|Rat CCL6 Gene cDNA clone plasmid||RG80721-U|
|Rat CCL6 natural ORF mammalian expression plasmid||RG80721-UT|
|Learn more about expression Vectors|
Chemokine (C-C motif) ligand 6 (CCL6), also known as C-C chemokine C10 has only been identified in rodents, which is a small cytokine belonging to the CC chemokine family, beta-chemokine subfamily. C-C chemokine C10 is involved in the chronic stages of host defense reactions. C10 chemokine rapidly promotes disease resolution in the cecal ligation and puncture (CLP) model through its direct effects on the cellular events critically involved in host defense during septic peritonitis. CCL6 appears to contribute to the macrophage infiltration that is independent of other CC chemokines. C10 is a prominent chemokine expressed in the central nervous system in experimental inflammatory demyelinating disease, also acts as a potent chemotactic factor for the migration of these leukocytes to the brain. CCL6 may be a mediator released by microglia for cell-cell communication under physiological as well as pathological conditions of CNS. Additionally, the chemokine CCL6 may alter tumor behavior by relieving its growth factor dependency and by promoting invasiveness as a result of local tissue apoptosis.