|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Recombinant Human Carbonic Anhydrase IV / CA4 protein (Catalog#10472-H08H)|
|0.2 μm filtered solution in PBS with 5% trehalose|
|Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase IV / CA4 (rh Carbonic Anhydrase IV / CA4; Catalog#10472-H08H; NP_000708.1; Met 1-Lys 283). Carbonic Anhydrase IV / CA4 specific IgG was purified by Human Carbonic Anhydrase IV / CA4 affinity chromatography.|
|Human Carbonic Anhydrase IV / CA4|
|WB, IHC-P, IP|
WB: 10-20 μg/mL
IHC-P: 0.5-2 μg/mL
IP: 4-8 μg/mg of lysate
|This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.