|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse PIK3IP1 ORF mammalian expression plasmid, C-GFPSpark tag||MG52429-ACG|
|Mouse PIK3IP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52429-ACR|
|Mouse PIK3IP1 ORF mammalian expression plasmid, C-Flag tag||MG52429-CF|
|Mouse PIK3IP1 ORF mammalian expression plasmid, C-His tag||MG52429-CH|
|Mouse PIK3IP1 ORF mammalian expression plasmid, C-Myc tag||MG52429-CM|
|Mouse PIK3IP1 ORF mammalian expression plasmid, C-HA tag||MG52429-CY|
|Mouse PIK3IP1 Gene cDNA clone plasmid||MG52429-G|
|Mouse PIK3IP1 ORF mammalian expression plasmid, N-Flag tag||MG52429-NF|
|Mouse PIK3IP1 ORF mammalian expression plasmid, N-His tag||MG52429-NH|
|Mouse PIK3IP1 ORF mammalian expression plasmid, N-Myc tag||MG52429-NM|
|Mouse PIK3IP1 ORF mammalian expression plasmid, N-HA tag||MG52429-NY|
|Mouse PIK3IP1 natural ORF mammalian expression plasmid||MG52429-UT|
|Learn more about expression Vectors|
PIK3IP1 contains 1 kringle domain and is a negative regulator of phosphatidylinositol-3-kinase (PI3K), suppresses the development of hepatocellular carcinoma. PI3K is a well-known regulator of cell division, motility, metabolism and survival in most cell types. Proper liver function and development highly depend on intact PI3K signal transduction. Aberrant PI3K pathway signaling in the liver is associated with hepatocellular carcinoma. PI3K signaling is involved in the homeostasis of lipid and glucose metabolism. Activation of the PI3K pathway induces lipogenesis and glycogenesis in the liver, since both Akt overexpressing transgenic mice and PTEN knockout mice develop fatty liver and hypoglycemia. PIK3IP1 overexpression can contribute to glucose homeostasis and fatty deposition.