|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat SELM ORF mammalian expression plasmid, C-GFPSpark tag||RG80607-ACG|
|Rat SELM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80607-ACR|
|Rat SELM ORF mammalian expression plasmid, C-Flag tag||RG80607-CF|
|Rat SELM ORF mammalian expression plasmid, C-His tag||RG80607-CH|
|Rat SELM ORF mammalian expression plasmid, C-Myc tag||RG80607-CM|
|Rat SELM ORF mammalian expression plasmid, C-HA tag||RG80607-CY|
|Rat SELM ORF mammalian expression plasmid, N-Flag tag||RG80607-NF|
|Rat SELM ORF mammalian expression plasmid, N-His tag||RG80607-NH|
|Rat SELM ORF mammalian expression plasmid, N-Myc tag||RG80607-NM|
|Rat SELM ORF mammalian expression plasmid, N-HA tag||RG80607-NY|
|Rat SELM Gene cDNA clone plasmid||RG80607-U|
|Rat SELM natural ORF mammalian expression plasmid||RG80607-UT|
|Learn more about expression Vectors|
Selenoprotein M is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine M is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This gene is expressed in a variety of tissues, and the protein is localized to the perinuclear structures. Selenoprotein M May function as a thiol-disulfide oxidoreductase that participates in disulfide bond formation. This protein is widely expressed and is highly expressed in brain. It is found in Cytoplasm, perinuclear region, Endoplasmic reticulum, Golgi apparatus. Localized to perinuclear structures corresponding to Golgi and endoplasmic reticulum. Experiments results have suggested that selenoprotein M may have an important role in protecting against oxidative damage in the brain and may potentially function in calcium regulation.