|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CASP14 ORF mammalian expression plasmid, C-GFPSpark tag||RG80602-ACG|
|Rat CASP14 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80602-ACR|
|Rat CASP14 ORF mammalian expression plasmid, N-GFPSpark tag||RG80602-ANG|
|Rat CASP14 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80602-ANR|
|Rat CASP14 ORF mammalian expression plasmid, C-Flag tag||RG80602-CF|
|Rat CASP14 ORF mammalian expression plasmid, C-His tag||RG80602-CH|
|Rat CASP14 ORF mammalian expression plasmid, C-Myc tag||RG80602-CM|
|Rat CASP14 ORF mammalian expression plasmid, C-HA tag||RG80602-CY|
|Rat CASP14 ORF mammalian expression plasmid, N-Flag tag||RG80602-NF|
|Rat CASP14 ORF mammalian expression plasmid, N-His tag||RG80602-NH|
|Rat CASP14 ORF mammalian expression plasmid, N-Myc tag||RG80602-NM|
|Rat CASP14 ORF mammalian expression plasmid, N-HA tag||RG80602-NY|
|Rat CASP14 Gene cDNA clone plasmid||RG80602-U|
|Rat CASP14 natural ORF mammalian expression plasmid||RG80602-UT|
|Learn more about expression Vectors|
Caspase 14 is a member of the caspase family. Caspases are a kind of cysteine proteinase consisting of a prodomain plus large and small catalytic subunits, that play a central role in cell apoptosis. Caspase 14 possesses an unusually short prodomain and is highly expressed in embryonic tissues but absent from most of the adult tissues except for the skin, which suggests a role in ontogenesis and skin physiology. Unlike the other short prodomain caspases(caspase-3, caspase-6, and caspase-7), Caspase 14 was not processed by multiple death stimuli including activation of members of the tumor necrosis factor receptor family and expression of proapaptotic members of the bcl-2 family. Caspase 14 has been described to be processed and activated by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may take part in keratinocyte terminal differentiation, which is essential for the skin barrier.