|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat STXBP3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80599-ACG|
|Rat STXBP3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80599-ACR|
|Rat STXBP3 ORF mammalian expression plasmid, N-GFPSpark tag||RG80599-ANG|
|Rat STXBP3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80599-ANR|
|Rat STXBP3 ORF mammalian expression plasmid, C-Flag tag||RG80599-CF|
|Rat STXBP3 ORF mammalian expression plasmid, C-His tag||RG80599-CH|
|Rat STXBP3 ORF mammalian expression plasmid, C-Myc tag||RG80599-CM|
|Rat STXBP3 ORF mammalian expression plasmid, C-HA tag||RG80599-CY|
|Rat STXBP3 ORF mammalian expression plasmid, N-Flag tag||RG80599-NF|
|Rat STXBP3 ORF mammalian expression plasmid, N-His tag||RG80599-NH|
|Rat STXBP3 ORF mammalian expression plasmid, N-Myc tag||RG80599-NM|
|Rat STXBP3 ORF mammalian expression plasmid, N-HA tag||RG80599-NY|
|Rat STXBP3 Gene cDNA clone plasmid||RG80599-U|
|Rat STXBP3 natural ORF mammalian expression plasmid||RG80599-UT|
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Syntaxin-binding protein 3, also known as Platelet Sec1 protein, Protein unc-18 homolog 3, Protein unc-18 homolog C, Unc-18C, Unc18-3 and STXBP3, is a cytoplasm protein which belongs to the STXBP/unc-18/SEC1 family. STXBP3 is expressed in cells that exhibit granule exocytosis, such as neutrophils, mast cells, platelets and endothelial cells. STXBP3, together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking / fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes. STXBP3 participates in the consolidation and secretion of secondary and tertiary granules. STXBP3 contains one SEC1 domain. Phosphorylation at Ser129 may stimulate granule release. Human STXBP3 shares 90% aa identity with mouse STXBP3. STXBP3 interacts with DOC2B; the interaction is direct, occurs at the cell membrane, excludes interaction with STX4 and regulates glucose-stimulated insulin secretion. Interacts with STX4.