|Datasheet||Specific References||Reviews||Related Products||Protocols|
|CST6, cystatin 6, cystatin M, cystatin M/E, cysteine proteinase inhibitor|
|A DNA sequence encoding the human cystatin E/M protein (NP_001314.1) (Met 1-Met 149) was expressed with a C-terminal polyhistidine-tag.|
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|> 95 % as determined by SDS-PAGE|
|Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate ZF RAMC, R&D Systems, Catalog # ES009 . IC50<2nM.|
|< 1.0 EU per μg of the protein as determined by the LAL method|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|The mature form of recombinant human cystatin E/M consists of 132 amino acids and predicts a molecular mass of 15 kDa. It exists as two major forms with an identical N-terminal sequence. As a result of glycosylation, rh cystatin E/M migrates as doublets with the molecular mass of approximately 15 and 21 kDa respectively in SDS-PAGE under reducing conditions.|
|Lyophilized from sterile 25mM MES, 0.15M NaCl, pH 6.5|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
Cystatin E/M, also referred to as CST6, is a member of type 2 cysteine proteinase inhibitors of the cystatin superfamily, and inhibits papain and cathepsin B. Cystatin E is a low molecular mass secreted protein existing in both a glycosylated (17 kDa) and an unglycosylated (14 kDa) form, with two characteristic intrachain disulfide bridges. Expression of cystatin M/E is found to be restricted to the epidermis, more specifically in the stratum granulosum, sweat glands, sebaceous glands, and the hair follicles. In addition to its function as a cysteine protease inhibitor, cystatin M/E also serves as a target for cross-linking by transglutaminases. Accordingly, cystatin M/E was suggested to be involved in barrier formation and maintenance. Furthermore, studies have revealed that cystatin M/E is frequently epigenetically inactivated during breast carcinogenesis, and thus be regarded as a candidate of tumour suppressor gene.