|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat ECM1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80574-ACG|
|Rat ECM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80574-ACR|
|Rat ECM1 ORF mammalian expression plasmid, C-Flag tag||RG80574-CF|
|Rat ECM1 ORF mammalian expression plasmid, C-His tag||RG80574-CH|
|Rat ECM1 ORF mammalian expression plasmid, C-Myc tag||RG80574-CM|
|Rat ECM1 ORF mammalian expression plasmid, C-HA tag||RG80574-CY|
|Rat ECM1 ORF mammalian expression plasmid, N-Flag tag||RG80574-NF|
|Rat ECM1 ORF mammalian expression plasmid, N-His tag||RG80574-NH|
|Rat ECM1 ORF mammalian expression plasmid, N-Myc tag||RG80574-NM|
|Rat ECM1 ORF mammalian expression plasmid, N-HA tag||RG80574-NY|
|Rat ECM1 Gene cDNA clone plasmid||RG80574-U|
|Rat ECM1 natural ORF mammalian expression plasmid||RG80574-UT|
|Learn more about expression Vectors|
Extracellular matrix protein 1 (ECM1) is a secreted glycoprotein and playing a pivotal role in endochondral bone formation, angiogenesis, and tumour biology. Three splice variants have been identified: ECM1a (540 aa) is most widely expressed, with highest expression in the placenta and heart; ECM1b (415 aa) is differentiation-dependent expressed and found only in tonsil and associated with suprabasal keratinocytes; ECM1c (559 aa) accounts for approximately 15% of skin ECM1. Although ECM1 is not tumor specific, is significantly elevated in many malignant epithelial tumors and is suggested as a possible trigger for angiogenesis, tumor progression and malignancies. It also has been shown to regulate endochondral bone formation, skeletal development and tissue remodeling.