|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat PKM ORF mammalian expression plasmid, C-GFPSpark tag||RG80568-ACG|
|Rat PKM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80568-ACR|
|Rat PKM ORF mammalian expression plasmid, N-GFPSpark tag||RG80568-ANG|
|Rat PKM ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80568-ANR|
|Rat PKM ORF mammalian expression plasmid, C-Flag tag||RG80568-CF|
|Rat PKM ORF mammalian expression plasmid, C-His tag||RG80568-CH|
|Rat PKM ORF mammalian expression plasmid, C-Myc tag||RG80568-CM|
|Rat PKM ORF mammalian expression plasmid, C-HA tag||RG80568-CY|
|Rat PKM ORF mammalian expression plasmid, N-Flag tag||RG80568-NF|
|Rat PKM ORF mammalian expression plasmid, N-His tag||RG80568-NH|
|Rat PKM ORF mammalian expression plasmid, N-Myc tag||RG80568-NM|
|Rat PKM ORF mammalian expression plasmid, N-HA tag||RG80568-NY|
|Rat PKM Gene cDNA clone plasmid||RG80568-U|
|Rat PKM natural ORF mammalian expression plasmid||RG80568-UT|
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Pyruvate kinase isozymes M2 also known as pyruvate kinase muscle isozyme 2 (PKM2), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an isoenzyme of the glycolytic enzyme pyruvate kinase. Pyruvate kinase isozymes M2 / PKM2 is a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. PKM2 has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. PKM2 functions as a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. PKM2 may stimulates POU5F1-mediated transcriptional activation. This protein Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms of PKM2 contributes to the control of glycolysis and is important for tumor cell proliferation and survival.