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TROP2 / TACSTD2 Antibody, Rabbit PAb, Antigen Affinity Purified

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Human TROP2/TACSTD2 Antibody Product Information
Immunogen:Recombinant Human TROP2 protein (Catalog#10428-H08H)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human TROP2 extracellular domain (rh TROP2; Catalog#10428-H08H; NP_002344.2; Met 1-Thr 274). TROP2 specific IgG was purified by human TROP2 affinity chromatography.
Human TROP2/TACSTD2 Antibody Usage Guide
Specificity:Human TROP2 / TACSTD2
Application:WB, ELISA, IHC-P, IP

WB: 10-20 μg/mL

ELISA: 0.5-1.0 μg/mL

This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human TROP2. The detection limit for human TROP2 is 0.00245 ng/well.

IHC-P: 0.1-2 μg/mL

IP: 1-4 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human TROP2/TACSTD2 Antibody WB Application Image
TROP2 / TACSTD2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Human TROP2/TACSTD2 Antibody IHC Application Image
[Click to enlarge image]
Caption:
Immunochemical staining of human TROP2 in human mammary gland with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of alveolus epithelium.
TROP2 / TACSTD2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human TROP2 in human mammary gland with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of alveolus epithelium.
TROP2 / TACSTD2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human TROP2 in human mammary gland with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of alveolus epithelium.
TROP2 / TACSTD2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human TROP2 in human placenta with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of cytotrophoblast.
TROP2 / TACSTD2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human TROP2 in human rectal cancer with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
TROP2 / TACSTD2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Human TROP2/TACSTD2 Antibody IP Application Image
TROP2 / TACSTD2 Antibody, Rabbit PAb, Antigen Affinity Purified
Other TROP2/TACSTD2 Antibody Products
TROP-2/TACSTD2 Background

TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.

Human TROP-2/TACSTD2 References
  • Ripani E, et al. (1998) Human Trop-2 is a tumor-associated calcium signal transducer. Int J Cancer. 76(5): 671-6.
  • Wang J, et al. (2008) Identification of Trop-2 as an oncogene and an attractive therapeutic target in colon cancers. Mol Cancer Ther. 7(2): 280-5.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"