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TROP2 / TACSTD2 Antibody, Mouse MAb

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TROP2/TACSTD2Antibody Product Information
Immunogen:Recombinant Human TROP2 protein (Catalog#10428-H08H)
Clone ID:01
Ig Type:Mouse IgG1
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TROP2 extracellular domain (rh TROP2; Catalog#10428-H08H; NP_002344.2; Met 1-Thr 274). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
TROP2/TACSTD2Antibody Usage Guide
Specificity:Human TROP2 / TACSTD2
No cross-reactivity in WB and ELISA with
Human BCAM / CD239
Human VCAM1 / CD106
Human ICAM1 / CD54
Human CD146 / MCAM / MUC18
Human CD171 / NCAML1 / L1CAM
Human Cadherin-1 / E-cadherin / CDH1 / CD324
Human cell lysate (293 cell line)
Application:ELISA, IHC-P, FCM

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human TROP2. The detection limit for Human TROP2 is 0.039 ng/well.

IHC-P: 5-15 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Images
TROP2 / TACSTD2 Antibody, Mouse MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human TROP2 in human placenta with mouse monoclonal antibody (15 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of cytotrophoblast.
TROP2 / TACSTD2 Antibody, Mouse MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human TROP2 in human mammary gland with mouse monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of alveolus epithelium.
TROP2 / TACSTD2 Antibody, Mouse MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human TROP2 in human rectal cancer with mouse monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
TROP-2 / TACSTD2 Antibody, Mouse MAb, Flow cytometric analysis
[Click to enlarge image]
Caption:
Flow cytometric analysis of human TROP2 expression on MCF-7 cells. Cells were stained with purified anti-Human TROP2, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Background

TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.

References
  • Ripani E, et al. (1998) Human Trop-2 is a tumor-associated calcium signal transducer. Int J Cancer. 76(5): 671-6.
  • Wang J, et al. (2008) Identification of Trop-2 as an oncogene and an attractive therapeutic target in colon cancers. Mol Cancer Ther. 7(2): 280-5.
  • Related Products
    Product nameProduct name
    Size / Price
    Catalog:10428-MM01-50
    List Price: $135.00  (Save $0.00)
    Price:$135.00      [How to order]
    AvailabilityIn Stock
    Images
    • TROP2 / TACSTD2 Antibody, Mouse MAb, Immunohistochemistry
    • TROP2 / TACSTD2 Antibody, Mouse MAb, Immunohistochemistry
    • TROP2 / TACSTD2 Antibody, Mouse MAb, Immunohistochemistry
    • TROP-2 / TACSTD2 Antibody, Mouse MAb, Flow cytometric analysis
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