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CD89 / FCAR Antibody, Mouse MAb

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Human CD89/FCAR Antibody Product Information
Immunogen:Recombinant Human CD89 protein (Catalog#10414-H08H)
Clone ID:6G3G3E7
Ig Type:Mouse IgG1
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD89 / FCAR (rh CD89; Catalog#10414-H08H; NP_001991.1; Met 1-Asn 227). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human CD89/FCAR Antibody Usage Guide
Specificity:Human CD89 / FCAR
No cross-reactivity with Human cell lysate (293 cell line) in WB and ELISA.
Application:ELISA, FCM

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CD89. The detection limit for Human CD89 is 0.31 ng/well.

FCM: 0.5-2 μg/Test

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CD89/FCAR Antibody FC Application Image
[Click to enlarge image]
Flow cytometric analysis of Human CD89 expression on Human blood granulocytes. Cells were stained with purified anti-Human CD89, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of viable granuolcytes.
CD89 / FCAR Antibody, Mouse MAb, Flow cytometric analysis
Other CD89/FCAR Antibody Products
CD89/FCAR Background

FCAR, also called FcαRI or CD89, is a type I  transmembrane receptor for Fc region of IgA which is the most abundant immunoglobulin in mucosal areas but is only the second most common antibody isotype in serum. This receptor is present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, especially phagocytes located in mucosal areas. Upon ligand IgA binding, FcαRI associates with the FcR γ signaling molecule bearing the immunoreceptor tyrosine-based activation motif (ITAM) through a unique charge-based mechanism and triggers multiple cell-mediated immune responses. It has been reported that Fc RI is a dual-function receptor that can mediate both inflammatory and anti-inflammatory responses depending on the type of interaction with its ligand. Sustained aggregation of FCAR results in activation of target-cell functions such as antigen presentation and cytokine release. In contrast, Monomeric targeting with serum IgA or with a variety of anti-FcαRI Fab fragments triggers an inhibitory response and additionally induces apoptosis. FcαRI thus play an fundamental role in preventing tumor development and growth, as well as in controlling inflammation.

Human CD89/FCAR References
  1. Maliszewski, C.R. et al., 1990, J. Exp. Med. 172: 1665-1672.
  2. Launay, P. et al., 1999, J. Biol. Chem. 274: 7216-7225.
  3. Monteiro, R.C. et al., 2003, Annu. Rev. Immunol. 21: 177-204.
  4. Otten, M.A. et al., 2005, J. Immunol. 174: 5472-5480.
  5. Pasquier, B. et al., 2005, Immunity. 22:31-42.
  6. Kanamaru, Y. et al., 2007, Blood. 109: 203-211.
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