|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|Mouse PLK1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50624-ACG|
|Mouse PLK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50624-ACR|
|Mouse PLK1 ORF mammalian expression plasmid, N-GFPSpark tag||MG50624-ANG|
|Mouse PLK1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50624-ANR|
|Mouse PLK1 ORF mammalian expression plasmid, C-Flag tag||MG50624-CF|
|Mouse PLK1 ORF mammalian expression plasmid, C-His tag||MG50624-CH|
|Mouse PLK1 ORF mammalian expression plasmid, C-Myc tag||MG50624-CM|
|Mouse PLK1 ORF mammalian expression plasmid, C-HA tag||MG50624-CY|
|Mouse PLK1 Gene cDNA clone plasmid||MG50624-M|
|Mouse PLK1 ORF mammalian expression plasmid, N-Flag tag||MG50624-NF|
|Mouse PLK1 ORF mammalian expression plasmid, N-His tag||MG50624-NH|
|Mouse PLK1 ORF mammalian expression plasmid, N-Myc tag||MG50624-NM|
|Mouse PLK1 ORF mammalian expression plasmid, N-HA tag||MG50624-NY|
|Mouse PLK1 natural ORF mammalian expression plasmid||MG50624-UT|
|Learn more about expression Vectors|
Serine / threonine-protein kinase PLK1 / PLK-1, also known as polo-like kinase 1 (PLK-1) or serine / threonine-protein kinase 13 (STPK13), Polo-like kinases (PLKs), is a family of four serine / threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1 / PLK-1 is ubiquitously expressed. The mRNA and protein expression of PLK1 / PLK-1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. PLK1 / PLK-1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 / PLK-1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 / PLK-1 overexpression correlates with cellular proliferation and poor prognosis. Serine / threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC / C inhibitors, and the regulation of mitotic exit and cytokinesis. It is required for recovery after DNA damage checkpoint and entry into mitosis. PLK1 / PLK-1 is required for kinetochore localization of BUB1B, spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. PLK1 / PLK-1 Phosphorylates BORA, and thereby promotes the degradation of BORA. PLK1 / PLK-1 also contributes to the regulation of AURKA function and phosphorylates SGOL1.