|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat PARK7 ORF mammalian expression plasmid, C-GFPSpark tag||RG80500-ACG|
|Rat PARK7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80500-ACR|
|Rat PARK7 ORF mammalian expression plasmid, N-GFPSpark tag||RG80500-ANG|
|Rat PARK7 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80500-ANR|
|Rat PARK7 ORF mammalian expression plasmid, C-Flag tag||RG80500-CF|
|Rat PARK7 ORF mammalian expression plasmid, C-His tag||RG80500-CH|
|Rat PARK7 ORF mammalian expression plasmid, C-Myc tag||RG80500-CM|
|Rat PARK7 ORF mammalian expression plasmid, C-HA tag||RG80500-CY|
|Rat PARK7 ORF mammalian expression plasmid, N-Flag tag||RG80500-NF|
|Rat PARK7 ORF mammalian expression plasmid, N-His tag||RG80500-NH|
|Rat PARK7 ORF mammalian expression plasmid, N-Myc tag||RG80500-NM|
|Rat PARK7 ORF mammalian expression plasmid, N-HA tag||RG80500-NY|
|Rat PARK7 Gene cDNA clone plasmid||RG80500-U|
|Rat PARK7 natural ORF mammalian expression plasmid||RG80500-UT|
|Learn more about expression Vectors|
Parkinson's disease locus DJ-1 (PARK7) is a differentially expressed transcript. DJ-1 plays a physiologic role in protection of erythroid cells from oxidant damage, a function unmasked in the context of oxidative stress. PARK7 belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Mutations in the DJ-1 gene are associated with rare forms of autosomal recessive early-onset Parkinson's disease (PD). DJ-1/p53 interactions contribute to apoptosis resistance in clonal myeloid cells and may serve as a prognostic marker in patients with myelodysplastic syndromes (MDS). DJ-1 regulates redox signaling kinase pathways and acts as a transcriptional regulator of antioxidative gene batteries. Therefore, DJ-1 is an important redox-reactive signaling intermediate controlling oxidative stress after ischemia, upon neuroinflammation, and during age-related neurodegenerative processes. Augmenting DJ-1 activity might provide novel approaches to treating chronic neurodegenerative illnesses such as Parkinson's disease and acute damage such as stroke.