|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CD5L ORF mammalian expression plasmid, C-GFPSpark tag||HG10791-ACG|
|Human CD5L ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10791-ACR|
|Human CD5L ORF mammalian expression plasmid, C-Flag tag||HG10791-CF|
|Human CD5L ORF mammalian expression plasmid, C-His tag||HG10791-CH|
|Human CD5L ORF mammalian expression plasmid, C-Myc tag||HG10791-CM|
|Human CD5L ORF mammalian expression plasmid, C-HA tag||HG10791-CY|
|Human CD5L Gene cDNA clone plasmid||HG10791-M|
|Human CD5L ORF mammalian expression plasmid, Flag tag||HG10791-M-F|
|Human CD5L ORF mammalian expression plasmid, N-Flag tag||HG10791-NF|
|Human CD5L ORF mammalian expression plasmid, N-His tag||HG10791-NH|
|Human CD5L ORF mammalian expression plasmid, N-Myc tag||HG10791-NM|
|Human CD5L ORF mammalian expression plasmid, N-HA tag||HG10791-NY|
|Human CD5L natural ORF mammalian expression plasmid||HG10791-UT|
|Learn more about expression Vectors|
CD5L, also known as CD5 antigen-like, is a soluble protein belonging to group B of the scavenger receptor cysteine-rich (SRCR) superfamily and contains three SRCR domains. It is a secreted glycoprotein and expressed by macrophages presentin lymphoid tissues (spleen, lymph node, thymus, and bone marrow). It binds to myelomonocytic and lymphoid cells and may play an important role in the regulation of the innate and adaptive immune systems. CD5L functions as a pattern recognition molecule by binding both lipoteichoic acid (LTA) on Gram positive and lipopolysaccharide (LPS) on Gram negative bacteria. and the SRCR domain 1 of CD5L retains both the LPS and LTA binding activities. In addtion, it is revealed that CD5L seems to play a role as an inhibitor of apoptosis.