|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat FABP7 ORF mammalian expression plasmid, C-GFPSpark tag||RG80474-ACG|
|Rat FABP7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80474-ACR|
|Rat FABP7 ORF mammalian expression plasmid, N-GFPSpark tag||RG80474-ANG|
|Rat FABP7 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80474-ANR|
|Rat FABP7 ORF mammalian expression plasmid, C-Flag tag||RG80474-CF|
|Rat FABP7 ORF mammalian expression plasmid, C-His tag||RG80474-CH|
|Rat FABP7 ORF mammalian expression plasmid, C-Myc tag||RG80474-CM|
|Rat FABP7 ORF mammalian expression plasmid, C-HA tag||RG80474-CY|
|Rat FABP7 ORF mammalian expression plasmid, N-Flag tag||RG80474-NF|
|Rat FABP7 ORF mammalian expression plasmid, N-His tag||RG80474-NH|
|Rat FABP7 ORF mammalian expression plasmid, N-Myc tag||RG80474-NM|
|Rat FABP7 ORF mammalian expression plasmid, N-HA tag||RG80474-NY|
|Rat FABP7 Gene cDNA clone plasmid||RG80474-U|
|Rat FABP7 natural ORF mammalian expression plasmid||RG80474-UT|
|Learn more about expression Vectors|
BLBP, also known as FABP7, is a brain fatty acid binding protein. Fatty acid binding proteins (FABPs) are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABP7 binds DHA with the highest affinity among all of the FABPs. FABPs may play roles in fatty acid uptake, transport, and metabolism. BLBP is expressed, during development, in radial glia by the activation of notch receptors. It was shown that reelin induces FABP7 expression in neural progenitor cells via notch-1 activation. BLBP variation is linked to weak prepulse inhibition(PPI) in mice and deficit in PPI is an endophenotypic trait observed in schizophrenia patients and their relatives.