|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL22 ORF mammalian expression plasmid, C-GFPSpark tag||RG80463-ACG|
|Rat IL22 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80463-ACR|
|Rat IL22 ORF mammalian expression plasmid, C-Flag tag||RG80463-CF|
|Rat IL22 ORF mammalian expression plasmid, C-His tag||RG80463-CH|
|Rat IL22 ORF mammalian expression plasmid, C-Myc tag||RG80463-CM|
|Rat IL22 ORF mammalian expression plasmid, C-HA tag||RG80463-CY|
|Rat IL22 Gene cDNA clone plasmid||RG80463-G|
|Rat IL22 ORF mammalian expression plasmid, N-Flag tag||RG80463-NF|
|Rat IL22 ORF mammalian expression plasmid, N-His tag||RG80463-NH|
|Rat IL22 ORF mammalian expression plasmid, N-Myc tag||RG80463-NM|
|Rat IL22 ORF mammalian expression plasmid, N-HA tag||RG80463-NY|
|Rat IL22 natural ORF mammalian expression plasmid||RG80463-UT|
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IL22 is a member of a group of cytokines called the IL-10 family or IL-10 superfamily (including IL-19, IL-20, IL-24, and IL-26), a class of potent mediators of cellular inflammatory responses. It shares use of IL-10R2 in cell signaling with other members of this family, IL-10, IL-26, IL-28A/B and IL-29. IL22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL22 along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems.
IL22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). IL22 and IL-10 receptor chains play a role in cellular targeting and signal transduction to selectively initiate and regulate immune responses. IL22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL22 are regulated by the often co-expressed cytokine IL-17A.