|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat SELP ORF mammalian expression plasmid, C-GFPSpark tag||RG80451-ACG|
|Rat SELP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80451-ACR|
|Rat SELP ORF mammalian expression plasmid, C-Flag tag||RG80451-CF|
|Rat SELP ORF mammalian expression plasmid, C-His tag||RG80451-CH|
|Rat SELP ORF mammalian expression plasmid, C-Myc tag||RG80451-CM|
|Rat SELP ORF mammalian expression plasmid, C-HA tag||RG80451-CY|
|Rat SELP Gene cDNA clone plasmid||RG80451-G|
|Rat SELP ORF mammalian expression plasmid, N-Flag tag||RG80451-NF|
|Rat SELP ORF mammalian expression plasmid, N-His tag||RG80451-NH|
|Rat SELP ORF mammalian expression plasmid, N-Myc tag||RG80451-NM|
|Rat SELP ORF mammalian expression plasmid, N-HA tag||RG80451-NY|
|Rat SELP natural ORF mammalian expression plasmid||RG80451-UT|
|Learn more about expression Vectors|
P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.