|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat PDCD1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80448-ACG|
|Rat PDCD1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80448-ACR|
|Rat PDCD1 ORF mammalian expression plasmid, C-Flag tag||RG80448-CF|
|Rat PDCD1 ORF mammalian expression plasmid, C-His tag||RG80448-CH|
|Rat PDCD1 ORF mammalian expression plasmid, C-Myc tag||RG80448-CM|
|Rat PDCD1 ORF mammalian expression plasmid, C-HA tag||RG80448-CY|
|Rat PDCD1 Gene cDNA clone plasmid||RG80448-G|
|Rat PDCD1 ORF mammalian expression plasmid, N-Flag tag||RG80448-NF|
|Rat PDCD1 ORF mammalian expression plasmid, N-His tag||RG80448-NH|
|Rat PDCD1 ORF mammalian expression plasmid, N-Myc tag||RG80448-NM|
|Rat PDCD1 ORF mammalian expression plasmid, N-HA tag||RG80448-NY|
|Rat PDCD1 natural ORF mammalian expression plasmid||RG80448-UT|
|Learn more about expression Vectors|
Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.