|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse NME1 ORF mammalian expression plasmid, C-GFPSpark tag||MG51576-ACG|
|Mouse NME1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51576-ACR|
|Mouse NME1 ORF mammalian expression plasmid, N-GFPSpark tag||MG51576-ANG|
|Mouse NME1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51576-ANR|
|Mouse NME1 ORF mammalian expression plasmid, C-Flag tag||MG51576-CF|
|Mouse NME1 ORF mammalian expression plasmid, C-His tag||MG51576-CH|
|Mouse NME1 ORF mammalian expression plasmid, C-Myc tag||MG51576-CM|
|Mouse NME1 ORF mammalian expression plasmid, C-HA tag||MG51576-CY|
|Mouse NME1 Gene cDNA clone plasmid||MG51576-G|
|Mouse NME1 ORF mammalian expression plasmid, N-Flag tag||MG51576-NF|
|Mouse NME1 ORF mammalian expression plasmid, N-His tag||MG51576-NH|
|Mouse NME1 ORF mammalian expression plasmid, N-Myc tag||MG51576-NM|
|Mouse NME1 ORF mammalian expression plasmid, N-HA tag||MG51576-NY|
|Mouse NME1 natural ORF mammalian expression plasmid||MG51576-UT|
|Learn more about expression Vectors|
NME1, also known as Nucleoside Diphosphate Kinase A (NDK-A), or NM23-H1, belongs to the NDK family. NM23-H1 is known to have a metastasis suppressive activity in many tumor cells. Recent studies have shown that the interacting proteins with NM23-H1 which mediate the cell proliferation, may act as modulators of the metastasis suppressor activity. The interacting proteins with NM23-H1 can be classified into 3 groups. The first group of proteins can be classified as upstream kinases of NM23-H1 such as CKI and Aurora-A/STK15. The second group of proteins acts as downstream effectors for the regulation of specific gene transcriptions, GTP-binding protein functions, and signal transduction in Erk signal cascade. The third group of proteins can be classified as bi-directionally influencing binding partners of NM23-H1. As a result, the interactions with NM23-H1 and binding partners have implications in the biochemical characterization involved in metastasis and tumorigenesis. NDKA is increased in human postmortem cerebrospinal fluid (CSF), a model of global brain insult, suggesting that measurement in CSF and, more importantly, in plasma may be useful as a biomarker of stroke. Additionally, NM23-H1 significantly reduces metastasis without effects on primary tumor size and was the first discovered metastasis suppressor gene.