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Thioredoxin / TXN Antibody, Rabbit PAb, Antigen Affinity Purified

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Human TXN Antibody Product Information
Immunogen:Recombinant Human Thioredoxin / TXN protein (Catalog#10384-HNAE)
Clone ID:
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human Thioredoxin / TXN (rh Thioredoxin / TXN; Catalog#10384-HNAE; P10599; Met 1-Val 105). Thioredoxin / TXN specific IgG was purified by Human Thioredoxin / TXN affinity chromatography.
Human TXN Antibody Usage Guide
Specificity:Human Thioredoxin / TXN
Application:WB, ELISA, IP

WB: 0.1-0.2 μg/mL

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human TXN. The detection limit for Human TXN is approximately 0.00245 ng/well.

IP: 0.5-1 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human TXN Antibody WB Application Image
Thioredoxin / TXN Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Thioredoxin / TXN Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Human TXN Antibody IP Application Image
Thioredoxin / TXN Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
Other TXN Antibody Products
Thioredoxin/TXN/SASP Background

Thioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein which belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Human Thioredoxin/TXN/SASP References
  • Holmgren A , et al.,1989, J Biol Chem 264 (24): 13963-6.
  • Nordberg J, et al., 2001, Free Radic Biol Med31 (11): 1287-312.
  • Mustacich D, et al., 2000, Biochem J 346 (Pt 1): 1-8. 
  • Mitchell D.A., et al., 2005, Nat. Chem. Biol. 1:154-158.
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