|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat LAIR1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80415-ACG|
|Rat LAIR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80415-ACR|
|Rat LAIR1 ORF mammalian expression plasmid, C-Flag tag||RG80415-CF|
|Rat LAIR1 ORF mammalian expression plasmid, C-His tag||RG80415-CH|
|Rat LAIR1 ORF mammalian expression plasmid, C-Myc tag||RG80415-CM|
|Rat LAIR1 ORF mammalian expression plasmid, C-HA tag||RG80415-CY|
|Rat LAIR1 Gene cDNA clone plasmid||RG80415-G|
|Rat LAIR1 ORF mammalian expression plasmid, N-Flag tag||RG80415-NF|
|Rat LAIR1 ORF mammalian expression plasmid, N-His tag||RG80415-NH|
|Rat LAIR1 ORF mammalian expression plasmid, N-Myc tag||RG80415-NM|
|Rat LAIR1 ORF mammalian expression plasmid, N-HA tag||RG80415-NY|
|Rat LAIR1 natural ORF mammalian expression plasmid||RG80415-UT|
|Learn more about expression Vectors|
Leukocyte associated Ig-like receptor-1 (LAIR1) is a surface molecule expressed on human mononuclear leukocytes that functions as an inhibitory receptor on human NK cells. In addition to NK cells, LAIR1 is expressed on T cells, B cells, macrophages, and dendritic cells. It is predicted to mediate inhibitory functions based on the presence of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic domain. Cross-linking of LAIR1 on human T cell clones results in inhibition of cytotoxicity only in T cell clones that lack CD28 and are able to spontaneously lyse certain targets in vitro. Moreover, the cytolytic activity of freshly isolated T cells, which is thought to be mainly due to "effector" T cells, can be inhibited by anti-LAIR1 mAb. Thus, LAIR1 functions as an inhibitory receptor not only on NK cells, but also on human T cells. This indicates that LAIR1 provides a mechanism of regulation of effector T cells and may play a role in the inhibition of unwanted bystander responses mediated by Ag-specific T cells.