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Human CD32a / FCGR2A Protein (167 His, His & AVI Tag), Biotinylated

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Human CD32a Protein Product Information
Synonym:RP11-5K23.6, CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A, FCGR2A1, FcGR, IGFR2, MGC23887, MGC30032
Protein Construction:A DNA sequence encoding the extracellular domain (Met 1-Met 210) of human CD32a (P12318-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. It is identical to FCGR2A131H/R in the reference.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human CD32a Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:1. Measured by its binding ability in a functional ELISA . Immobilized human IgG2 at 10 μg/ml (100 μl/well) can bind biotinylated human CD32 with a linear ranger of 0.16-0.8 μg/ml.
2. Using the Octet RED System, the affinity constant (Kd) of CD32a-AVI-His + BirA bound to human IgG was 12nM .
3. Labeling ratio of biotin to protein: 0.1-0.6.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Gln 34
Molecule Mass:The recombinant human CD32aconsists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.6 KDa. It migrates as an approximately 33 KDa band due to glycosylation by SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human CD32a Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human CD32a Protein SDS-PAGE
Human CD32a / FCGR2A Protein (167 His, His & AVI Tag), Biotinylated SDS-PAGE
Other CD32a Recombinant Protein Products
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Catalog: 10374-H27H1-B-20
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