|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CNTN5 ORF mammalian expression plasmid, C-GFPSpark tag||RG80409-ACG|
|Rat CNTN5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80409-ACR|
|Rat CNTN5 ORF mammalian expression plasmid, C-Flag tag||RG80409-CF|
|Rat CNTN5 ORF mammalian expression plasmid, C-His tag||RG80409-CH|
|Rat CNTN5 ORF mammalian expression plasmid, C-Myc tag||RG80409-CM|
|Rat CNTN5 ORF mammalian expression plasmid, C-HA tag||RG80409-CY|
|Rat CNTN5 Gene cDNA clone plasmid||RG80409-G|
|Rat CNTN5 ORF mammalian expression plasmid, N-Flag tag||RG80409-NF|
|Rat CNTN5 ORF mammalian expression plasmid, N-His tag||RG80409-NH|
|Rat CNTN5 ORF mammalian expression plasmid, N-Myc tag||RG80409-NM|
|Rat CNTN5 ORF mammalian expression plasmid, N-HA tag||RG80409-NY|
|Rat CNTN5 natural ORF mammalian expression plasmid||RG80409-UT|
|Learn more about expression Vectors|
Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed mainly in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2(TAG-1), Contactin-3(BIG-1), BIG-2, Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-5, also known as NB-2, is one of the neural recognition molecules in the contactin subgroup. Contactin-5 is expressed in brain and kidney and at very low level in placenta. In brain, Contactin-5 is highly expressed in the occipital lobe, amygdala, cerebral cortex, frontal lobe, thalamus and temporal lobe. Mice deficient in the Contactin-5 gene exhibit aberrant responses to acoustic stimuli. Contactin-5 may play a role in maturation of glutamatergic synapses in the brainstem during the final stages of auditory development. Contactin-5 gene may contribute to human neurological disorders.