|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat NCAM1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80399-ACG|
|Rat NCAM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80399-ACR|
|Rat NCAM1 ORF mammalian expression plasmid, C-Flag tag||RG80399-CF|
|Rat NCAM1 ORF mammalian expression plasmid, C-His tag||RG80399-CH|
|Rat NCAM1 ORF mammalian expression plasmid, C-Myc tag||RG80399-CM|
|Rat NCAM1 ORF mammalian expression plasmid, C-HA tag||RG80399-CY|
|Rat NCAM1 Gene cDNA clone plasmid||RG80399-G|
|Rat NCAM1 ORF mammalian expression plasmid, N-Flag tag||RG80399-NF|
|Rat NCAM1 ORF mammalian expression plasmid, N-His tag||RG80399-NH|
|Rat NCAM1 ORF mammalian expression plasmid, N-Myc tag||RG80399-NM|
|Rat NCAM1 ORF mammalian expression plasmid, N-HA tag||RG80399-NY|
|Rat NCAM1 natural ORF mammalian expression plasmid||RG80399-UT|
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NCAM1, also known as CD56, is a neural adhesion protein (NCAM) which belongs to the immunoglobulin superfamily. NCAM is involved in neural development and in plasticity in the adult brain. UCHL1 is a novel interaction partner of both NCAM isoforms that regulates their ubiquitination and intracellular trafficking. NCAM1 is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. NCAM1 has also been shown to be involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.