|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat ENPEP ORF mammalian expression plasmid, C-GFPSpark tag||RG80387-ACG|
|Rat ENPEP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80387-ACR|
|Rat ENPEP ORF mammalian expression plasmid, C-Flag tag||RG80387-CF|
|Rat ENPEP ORF mammalian expression plasmid, C-His tag||RG80387-CH|
|Rat ENPEP ORF mammalian expression plasmid, C-Myc tag||RG80387-CM|
|Rat ENPEP ORF mammalian expression plasmid, C-HA tag||RG80387-CY|
|Rat ENPEP Gene cDNA clone plasmid||RG80387-G|
|Rat ENPEP ORF mammalian expression plasmid, N-Flag tag||RG80387-NF|
|Rat ENPEP ORF mammalian expression plasmid, N-His tag||RG80387-NH|
|Rat ENPEP ORF mammalian expression plasmid, N-Myc tag||RG80387-NM|
|Rat ENPEP ORF mammalian expression plasmid, N-HA tag||RG80387-NY|
|Rat ENPEP natural ORF mammalian expression plasmid||RG80387-UT|
|Learn more about expression Vectors|
ENPEP, also known as aminopeptidase A, is a member of the peptidase M1 family. Members of this family are involved in response to cadmium ion and proteolysis. They located in 6 components and are expressed in 26 plant structures. ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It also can be detected in a variety of other tissues. ENPEP probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. ENPEP is a zinc-dependent membrane-bound aminopeptidase that catalyzes the cleavage of glutamatic and aspartatic amino acid residues from the N-terminus of polypeptides. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure.