|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TRIB2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10725-ACG|
|Human TRIB2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10725-ACR|
|Human TRIB2 ORF mammalian expression plasmid, N-GFPSpark tag||HG10725-ANG|
|Human TRIB2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10725-ANR|
|Human TRIB2 ORF mammalian expression plasmid, C-Flag tag||HG10725-CF|
|Human TRIB2 ORF mammalian expression plasmid, C-His tag||HG10725-CH|
|Human TRIB2 ORF mammalian expression plasmid, C-Myc tag||HG10725-CM|
|Human TRIB2 ORF mammalian expression plasmid, C-HA tag||HG10725-CY|
|Human TRIB2 Gene cDNA clone plasmid||HG10725-M|
|Human TRIB2 ORF mammalian expression plasmid, Flag tag||HG10725-M-F|
|Human TRIB2 ORF mammalian expression plasmid, N-Flag tag||HG10725-NF|
|Human TRIB2 ORF mammalian expression plasmid, N-His tag||HG10725-NH|
|Human TRIB2 ORF mammalian expression plasmid, N-Myc tag||HG10725-NM|
|Human TRIB2 ORF mammalian expression plasmid, N-HA tag||HG10725-NY|
|Human TRIB2 natural ORF mammalian expression plasmid||HG10725-UT|
|Learn more about expression Vectors|
Tribbles homolog 2, also known as TRB-2, and Trib2, is a member of the protein kinase superfamily and Tribbles subfamily (Trib1, Trib2, Trib3). The identification of tribbles as regulators of signal processing systems and physiological processes, including development, together with their potential involvement in diabetes and cancer, has generated considerable interest in these proteins. Tribbles have been reported to regulate activation of a number of intracellular signalling pathways with roles extending from mitosis and cell activation to apoptosis and modulation of gene expression. Tribbles controls the timing of mitosis in the prospective mesoderm, allowing cell-shape changes to be completed. This mechanism for coordinating cell division and cell-shape changes may have helped Drosophila to evolve its mode of rapid early development. Trib2 was identified as a downregulated transcript in leukemic cells undergoing growth arrest. Trib2-transduced bone marrow cells exhibited a growth advantage and readily established factor-dependent cell lines. Trib2-reconstituted mice uniformly developed fatal transplantable acute myelogenous leukemia (AML).