|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Recombinant Human HSP27 protein (Catalog#10351-H08E)|
|0.2 μm filtered solution in PBS with 5% trehalose|
|Produced in rabbits immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met 1-Lys 205). HSP27 specific IgG was purified by Human HSP27 affinity chromatography.|
|WB, ELISA, IHC-P, IP|
WB: 2-5 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human HSP27. The detection limit for Human HSP27 is approximately 0.00245 ng/well.
IHC-P: 0.05-1 μg/mL
IP: 0.5-2 μg/mg of lysate
|This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.|
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.