|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat THBD ORF mammalian expression plasmid, C-GFPSpark tag||RG80348-ACG|
|Rat THBD ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80348-ACR|
|Rat THBD ORF mammalian expression plasmid, C-Flag tag||RG80348-CF|
|Rat THBD ORF mammalian expression plasmid, C-His tag||RG80348-CH|
|Rat THBD ORF mammalian expression plasmid, C-Myc tag||RG80348-CM|
|Rat THBD ORF mammalian expression plasmid, C-HA tag||RG80348-CY|
|Rat THBD Gene cDNA clone plasmid||RG80348-G|
|Rat THBD ORF mammalian expression plasmid, N-Flag tag||RG80348-NF|
|Rat THBD ORF mammalian expression plasmid, N-His tag||RG80348-NH|
|Rat THBD ORF mammalian expression plasmid, N-Myc tag||RG80348-NM|
|Rat THBD ORF mammalian expression plasmid, N-HA tag||RG80348-NY|
|Rat THBD natural ORF mammalian expression plasmid||RG80348-UT|
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Thrombomodulin, also known as THBD(CD141), is an integral membrane protein which reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. Thrombomodulin also regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene have also been reported to be associated with atypical hemolytic-uremic syndrome.