|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat SDC1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80344-ACG|
|Rat SDC1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80344-ACR|
|Rat SDC1 ORF mammalian expression plasmid, C-Flag tag||RG80344-CF|
|Rat SDC1 ORF mammalian expression plasmid, C-His tag||RG80344-CH|
|Rat SDC1 ORF mammalian expression plasmid, C-Myc tag||RG80344-CM|
|Rat SDC1 ORF mammalian expression plasmid, C-HA tag||RG80344-CY|
|Rat SDC1 Gene cDNA clone plasmid||RG80344-G|
|Rat SDC1 ORF mammalian expression plasmid, N-Flag tag||RG80344-NF|
|Rat SDC1 ORF mammalian expression plasmid, N-His tag||RG80344-NH|
|Rat SDC1 ORF mammalian expression plasmid, N-Myc tag||RG80344-NM|
|Rat SDC1 ORF mammalian expression plasmid, N-HA tag||RG80344-NY|
|Rat SDC1 natural ORF mammalian expression plasmid||RG80344-UT|
|Learn more about expression Vectors|
Syndecan-1 also known as SDC1 and CD138, is the most extensively studied member of the syndecan family. It is found mainly in epithelial cells, but its expression is developmentally regulated during embryonic development. Syndecan-1/SDC1/CD138 has been shown to mediate cell adhesion to several ECM molecules, and to act as a coreceptor for fibroblast growth factors, potent angiogenic growth factors involved also in differentiation. Syndecan-1/SDC1/CD138 expression is reduced during malignant transformation of various epithelia, and this loss correlates with the histological differentiation grade of squamous cell carcinomas, lacking from poorly differentiated tumours. In squamous cell carcinomas of the head and neck, positive syndecan-1 expression correlates with a more favourable prognosis. Experimental studies on the role of Syndecan-1 in malignant transformation have shown that Syndecan-1/SDC1/CD138 expression is associated with the maintenance of epithelial morphology, anchorage-dependent growth and inhibition of invasiveness in vitro.