|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse RAMP3 ORF mammalian expression plasmid, C-GFPSpark tag||MG51528-ACG|
|Mouse RAMP3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51528-ACR|
|Mouse RAMP3 ORF mammalian expression plasmid, C-Flag tag||MG51528-CF|
|Mouse RAMP3 ORF mammalian expression plasmid, C-His tag||MG51528-CH|
|Mouse RAMP3 ORF mammalian expression plasmid, C-Myc tag||MG51528-CM|
|Mouse RAMP3 ORF mammalian expression plasmid, C-HA tag||MG51528-CY|
|Mouse RAMP3 Gene cDNA clone plasmid||MG51528-G|
|Mouse RAMP3 ORF mammalian expression plasmid, N-Flag tag||MG51528-NF|
|Mouse RAMP3 ORF mammalian expression plasmid, N-His tag||MG51528-NH|
|Mouse RAMP3 ORF mammalian expression plasmid, N-Myc tag||MG51528-NM|
|Mouse RAMP3 ORF mammalian expression plasmid, N-HA tag||MG51528-NY|
|Mouse RAMP3 natural ORF mammalian expression plasmid||MG51528-UT|
|Learn more about expression Vectors|
RAMP3 belongs to the RAMP family. Members of this family are single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs have a wide biological distribution; high concentrations are found in the brain, lung, liver, heart and spleen with lower expression levels present in the testes, gastrointestinal tract and thyroid. RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. They are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of RAMP3 protein, CRLR functions as an adrenomedullin receptor.