|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CNTN3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80316-ACG|
|Rat CNTN3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80316-ACR|
|Rat CNTN3 ORF mammalian expression plasmid, C-Flag tag||RG80316-CF|
|Rat CNTN3 ORF mammalian expression plasmid, C-His tag||RG80316-CH|
|Rat CNTN3 ORF mammalian expression plasmid, C-Myc tag||RG80316-CM|
|Rat CNTN3 ORF mammalian expression plasmid, C-HA tag||RG80316-CY|
|Rat CNTN3 Gene cDNA clone plasmid||RG80316-G|
|Rat CNTN3 ORF mammalian expression plasmid, N-Flag tag||RG80316-NF|
|Rat CNTN3 ORF mammalian expression plasmid, N-His tag||RG80316-NH|
|Rat CNTN3 ORF mammalian expression plasmid, N-Myc tag||RG80316-NM|
|Rat CNTN3 ORF mammalian expression plasmid, N-HA tag||RG80316-NY|
|Rat CNTN3 natural ORF mammalian expression plasmid||RG80316-UT|
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Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2(TAG-1), Contactin-3(BIG-1), BIG-2, Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-3, also known as CNTN3 ( BIG-1 in rat and PANG in mouse ), is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. In the brain, Contactin-3 is expressed in frontal lobe, occipital lobe, cerebellum and amygdala. Contactin-3 contains 4 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. Human Contactin-3 shares 92% aa identity with mouse Contactin-3.The exact function of Contactin-3 is unclear. Contactin-3 may mediate cell-cell interaction and may promote neurite outgrowth.