|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CD68 ORF mammalian expression plasmid, C-GFPSpark tag||RG80307-ACG|
|Rat CD68 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80307-ACR|
|Rat CD68 ORF mammalian expression plasmid, C-Flag tag||RG80307-CF|
|Rat CD68 ORF mammalian expression plasmid, C-His tag||RG80307-CH|
|Rat CD68 ORF mammalian expression plasmid, C-Myc tag||RG80307-CM|
|Rat CD68 ORF mammalian expression plasmid, C-HA tag||RG80307-CY|
|Rat CD68 Gene cDNA clone plasmid||RG80307-G|
|Rat CD68 ORF mammalian expression plasmid, N-Flag tag||RG80307-NF|
|Rat CD68 ORF mammalian expression plasmid, N-His tag||RG80307-NH|
|Rat CD68 ORF mammalian expression plasmid, N-Myc tag||RG80307-NM|
|Rat CD68 ORF mammalian expression plasmid, N-HA tag||RG80307-NY|
|Rat CD68 natural ORF mammalian expression plasmid||RG80307-UT|
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Macrosialin, also known as CD68 and Gp110, is a single-pass type I membrane protein which belongs to the LAMP family. CD68 is highly expressed by blood monocytes and tissue macrophages. It is also expressed in lymphocytes, fibroblasts and endothelial cells. CD68 is expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It is a commonly used marker for macrophages. However, a number of studies have shown that CD68 antibodies react with other hematopoietic and non-hematopoietic cell types, suggesting that CD68 may not be a macrophage-specific antigen. CD68 binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.