|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TNFRSF17 ORF mammalian expression plasmid, C-GFPSpark tag||HG10620-ACG|
|Human TNFRSF17 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10620-ACR|
|Human TNFRSF17 ORF mammalian expression plasmid, N-GFPSpark tag||HG10620-ANG|
|Human TNFRSF17 ORF mammalian expression plasmid, C-Flag tag||HG10620-CF|
|Human TNFRSF17 ORF mammalian expression plasmid, C-His tag||HG10620-CH|
|Human TNFRSF17 ORF mammalian expression plasmid, C-Myc tag||HG10620-CM|
|Human TNFRSF17 ORF mammalian expression plasmid, C-HA tag||HG10620-CY|
|Human TNFRSF17 Gene cDNA clone plasmid||HG10620-M|
|Human TNFRSF17 ORF mammalian expression plasmid, N-Flag tag||HG10620-NF|
|Human TNFRSF17 ORF mammalian expression plasmid, N-His tag||HG10620-NH|
|Human TNFRSF17 ORF mammalian expression plasmid, N-Myc tag||HG10620-NM|
|Human TNFRSF17 ORF mammalian expression plasmid, N-HA tag||HG10620-NY|
|Human TNFRSF17 natural ORF mammalian expression plasmid||HG10620-UT|
|Learn more about expression Vectors|
Tumor necrosis factor receptor superfamily, member 17 (TNFRSF17), also known as B cell maturation antigen (BCMA) or CD269 antigen, is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13BBAFF), and to lead to NF-kappaB and MAPK8/JNK activation. TNFRSF17/BCMA/CD269 also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. TNFRSF17/BCMA/CD269 is a receptor for TALL-1 and BCMA activates NF-kappaB through a TRAF5-, TRAF6-, NIK-, and IKK-dependent pathway. The identification of TNFRSF17 as a NF-kappaB-activating receptor for TALL-1 suggests molecular targets for drug development against certain immunodeficient or autoimmune diseases. TNFRSF17/BCMA is a target of donor B-cell immunity in patients with myeloma who respond to DLI. Antibody responses to cell-surface BCMA may contribute directly to tumor rejection in vivo.