|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FGFR1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10616-ACG|
|Human FGFR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10616-ACR|
|Human FGFR1 ORF mammalian expression plasmid, C-Flag tag||HG10616-CF|
|Human FGFR1 ORF mammalian expression plasmid, C-His tag||HG10616-CH|
|Human FGFR1 ORF mammalian expression plasmid, C-Myc tag||HG10616-CM|
|Human FGFR1 ORF mammalian expression plasmid, C-HA tag||HG10616-CY|
|Human FGFR1 Gene cDNA clone plasmid||HG10616-M|
|Human FGFR1 ORF mammalian expression plasmid, N-Flag tag||HG10616-NF|
|Human FGFR1 ORF mammalian expression plasmid, N-His tag||HG10616-NH|
|Human FGFR1 ORF mammalian expression plasmid, N-Myc tag||HG10616-NM|
|Human FGFR1 ORF mammalian expression plasmid, N-HA tag||HG10616-NY|
|Human FGFR1 natural ORF mammalian expression plasmid||HG10616-UT|
|Learn more about expression Vectors|
FGFR1, also known as CD331, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. Fibroblast growth factors (FGFs) (FGF1 - 10 and 16 - 23) are mitogenic signaling molecules that have roles in angiogenesis, wound healing, cell migration, neural outgrowth and embryonic development. FGFs bind heparan sulfate glycosaminoglycans, which facilitates dimerization (activation) of FGF receptors. FGFR1 is a full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR1 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. CD331 can be detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. Defects in FGFR1 are a cause of Pfeiffer syndrome ，idiopathic hypogonadotropic hypogonadism， Kallmann syndrome type 2, osteoglophonic dysplasia and trigonocephaly non-syndromic.