|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CDH15 ORF mammalian expression plasmid, C-GFPSpark tag||RG80281-ACG|
|Rat CDH15 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80281-ACR|
|Rat CDH15 ORF mammalian expression plasmid, C-Flag tag||RG80281-CF|
|Rat CDH15 ORF mammalian expression plasmid, C-His tag||RG80281-CH|
|Rat CDH15 ORF mammalian expression plasmid, C-Myc tag||RG80281-CM|
|Rat CDH15 ORF mammalian expression plasmid, C-HA tag||RG80281-CY|
|Rat CDH15 Gene cDNA clone plasmid||RG80281-G|
|Rat CDH15 ORF mammalian expression plasmid, N-Flag tag||RG80281-NF|
|Rat CDH15 ORF mammalian expression plasmid, N-His tag||RG80281-NH|
|Rat CDH15 ORF mammalian expression plasmid, N-Myc tag||RG80281-NM|
|Rat CDH15 ORF mammalian expression plasmid, N-HA tag||RG80281-NY|
|Rat CDH15 natural ORF mammalian expression plasmid||RG80281-UT|
|Learn more about expression Vectors|
Cadherin-15, also known as CDH15, is a member of the cadherin superfamily. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherin-15 contains 5 cadherin domains. It is expressed in some normal epithelial tissues and in some carcinoma cell lines. Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM), also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly.