|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CDH12 ORF mammalian expression plasmid, C-GFPSpark tag||RG80279-ACG|
|Rat CDH12 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80279-ACR|
|Rat CDH12 ORF mammalian expression plasmid, C-Flag tag||RG80279-CF|
|Rat CDH12 ORF mammalian expression plasmid, C-His tag||RG80279-CH|
|Rat CDH12 ORF mammalian expression plasmid, C-Myc tag||RG80279-CM|
|Rat CDH12 ORF mammalian expression plasmid, C-HA tag||RG80279-CY|
|Rat CDH12 Gene cDNA clone plasmid||RG80279-G|
|Rat CDH12 ORF mammalian expression plasmid, N-Flag tag||RG80279-NF|
|Rat CDH12 ORF mammalian expression plasmid, N-His tag||RG80279-NH|
|Rat CDH12 ORF mammalian expression plasmid, N-Myc tag||RG80279-NM|
|Rat CDH12 ORF mammalian expression plasmid, N-HA tag||RG80279-NY|
|Rat CDH12 natural ORF mammalian expression plasmid||RG80279-UT|
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Classic Cadherins represent a family of calcium-dependent homophilic cell-cell adhesion molecules. They confer strong adhesiveness to animal cells when they are anchored to the actin cytoskeleton via their cytoplasmic binding partners, catenins. The cadherin/catenin adhesion system plays key roles in the morphogenesis and function of the vertebrate and invertebrate nervous systems. Furthermore, this system is involved in synaptic plasticity. Recent studies on the role of individual cadherin subtypes at synapses indicate that individual cadherin subtypes play their own unique role to regulate synaptic activities. Type II (atypical) cadherins are defined based on their lack of an HAV cell adhesion recognition sequence specific to type I cadherins. It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development. Cadherin-12 also known as CDH12, is a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherin-12 appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis.